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Researchers from multiple institutions used quantitative molecular diagnostic methods to reassess causes of diarrhea in children who had participated in the Global Enteric Multi-center Study (GEMS). GEMS was designed to investigate moderate-to-severe diarrhea in children younger than 5 years in countries in Africa and Asia. As part of GEMS, traditional microbiologic techniques were used to identify pathogens in cases (children with diarrhea) and matched controls (children without diarrhea). For the current study, quantitative real-time PCR was used to test for 32 enteropathogens in stool samples from a subset of cases and matched controls. The attributable incidence of pathogen-associated diarrhea was calculated by …
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